Method development and validation of cabozantinib by LC-MS/MS

The objective of this method is to be simple, precise, and economical performed by LC-MS/MS instrument. mass spectrometric determination was using electrospray ionization in the positive mode with multiple reaction monitoring (MRM) precursor product ion transition m/z 502.2 > 323 for cabozantinib. effective separation cabozantinib achieved X-Bridge (2.1 mm × 100 mm, 3.5 µ) column mobile phase composition 0.2% formic acid: acetonitrile (40:60 v/v), pumped at 0.12 ml/min flow rate. Rt found 1.34 minutes. LOD LOQ were 1.5 ng/ml 5 concentrations linearity a range 75 regression correlation coefficient 0.999. % RSD value accuracy observed 1.2–2.0. marketed formulation assay 99.82%. developed validation parameters accepted as per USFDA guidelines.